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mc 050 fixation buffer biolegend  (R&D Systems)


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    R&D Systems mc 050 fixation buffer biolegend
    Mc 050 Fixation Buffer Biolegend, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+madcam+1+fc+chimera/Recombinant+Mouse+MAdCAM-1+Fc+Chimera+Protein%2C+CF/pm41964950-520-43-40
    Average 93 stars, based on 16 article reviews
    mc 050 fixation buffer biolegend - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Cell Adhesion Assay:

    Article Title: Multifunctional Activity of a Small Tellurium Redox Immunomodulator Compound, AS101, on Dextran Sodium Sulfate-induced Murine Colitis
    Article Snippet: Blots were developed using horseradish peroxidase-conjugated secondary antibodies and the ECL detection system (Pierce). .. Adhesion Assay of MLN Cells to MAdCAM-1 A 96-well plate was coated with 80 μl/well of 5μg/ml recombinant mouse MAdCAM-1 Fc chimera (R&D Systems). .. For the in vitro assay, control wells were coated with 2.5% BSA (Sigma).

    Article Title: Multifunctional Activity of a Small Tellurium Redox Immunomodulator Compound, AS101, on Dextran Sodium Sulfate-induced Murine Colitis
    Article Snippet: Blots were developed using horseradish peroxidaseconjugated secondary antibodies and the ECL detection system (Pierce). .. Adhesion assay of MLN cells to MAdCAM-1 A 96-well plate was coated with 80ul/well of 5μgr/ml recombinant mouse MAdCAM-1 Fc chimera (R&D systems). .. For the in vitro assay, control wells were coated with 2.5% BSA (Sigma).

    Recombinant:

    Article Title: Multifunctional Activity of a Small Tellurium Redox Immunomodulator Compound, AS101, on Dextran Sodium Sulfate-induced Murine Colitis
    Article Snippet: Blots were developed using horseradish peroxidase-conjugated secondary antibodies and the ECL detection system (Pierce). .. Adhesion Assay of MLN Cells to MAdCAM-1 A 96-well plate was coated with 80 μl/well of 5μg/ml recombinant mouse MAdCAM-1 Fc chimera (R&D Systems). .. For the in vitro assay, control wells were coated with 2.5% BSA (Sigma).

    Article Title: Homodetic cyclic peptides targeting alpha-4-beta-7 integrin
    Article Snippet: Integrin α4β7-MAdCAM Mouse Cell Adhesion Assay TK-1 mouse T-cells lymphoma cell lines (ATCC CRL2396) were cultured in RPMI 1640 medium (HyClone SH30027.1) supplemented with 10% FBS (Seradigm) and 1% Penicillin-Streptomycin. .. A 96-well plate (Costar, 3603) was coated with 100 μl/well of recombinant mouse MAdCAM-1 Fc Chimera (R&D Systems, 993-MC-050) solution at 0.25 μg/ml in coating buffer (50 mM sodium carbonate, pH 9.6). ..

    Article Title: Antibodies with improved stability to intestinal digestion, polynucleotides thereof and methods of use thereof to treat disease
    Article Snippet: Gliadin 33-mer (H-Leu-Gln-Leu-Gln-Pro-Phe-Pro-Gln-Pro-Gln-Leu-Pro-Tyr-Pro-Gln-Pro-Gln-Leu-Pro-Tyr-Pro-Gln-Pro-Gln-Leu-Pro-Tyr-Pro-Gln-Pro-Gln-Pro-Phe-NH2; SEQ ID NO: 25) peptide was synthesized by Bachem (Torrance, CA, catalog #4077401) and represents residues 56-88 in the gliadin molecule (Shan et al., 2002). .. Other protein antigens were: Recombinant Mouse IL-6Ra (carrier free) from R & D Systems (Cat #1830-SR/CF, lot #ITY141301A; Minneapolis, MN); Recombinant Mouse IL-6 from Cell Sciences (Cat #CRI130C, lot #3201610; Canton, MA); Carrier Free Recombinant Mouse IL-12/IL-23 p40 Homodimer from R& D Systems (Cat #499-ML/CF, lot #RH041212A; Minneapolis, MN) and Recombinant Mouse MAdCAM-1 Fc Chimera from R & D Systems (Cat #993-MC-050, lot #E0H1312101; Minneapolis, MN). ..

    Article Title: Multifunctional Activity of a Small Tellurium Redox Immunomodulator Compound, AS101, on Dextran Sodium Sulfate-induced Murine Colitis
    Article Snippet: Blots were developed using horseradish peroxidaseconjugated secondary antibodies and the ECL detection system (Pierce). .. Adhesion assay of MLN cells to MAdCAM-1 A 96-well plate was coated with 80ul/well of 5μgr/ml recombinant mouse MAdCAM-1 Fc chimera (R&D systems). .. For the in vitro assay, control wells were coated with 2.5% BSA (Sigma).

    Article Title: Tellurium Compounds Prevent and Reverse Type-1 Diabetes in NOD Mice by Modulating α4β7 Integrin Activity, IL-1β, and T Regulatory Cells
    Article Snippet: .. FITC anti-mouse CD3; PE anti-human/mouse Integrin β7 (Biolegend, San Diego, CA, USA); APC anti-mouse CD45R/B220; anti-Mouse/Rat Foxp3 PE; anti-Mouse CD4 FITC; anti-Mouse CD25 APC; rat IgG2a Isotype Control PE (eBioscience, Carlsbad, CA, USA); Alexa 594 anti-mouse; Alexa 488 anti-rabbit; anti-glucagon; anti-insulin (Innovex Biosciences, Richmond, CA, USA); recombinant mouse MAdCAM-1 Fc chimera (R&D BioSystems, Minneapolis, MN, USA); murine TNF-α (Peprotech, Rocky Hill, NJ, USA); mouse IL-1β, IFNγ, and IL-17 ELISA kits (R&D Biosystems). .. AS101 and SAS were supplied by M. Albeck, Bar-Ilan University, Ramat-Gan, Israel, and were synthesized at Bar-Ilan University.

    Article Title: Multifunctional Activity of a Small Tellurium Redox Immunomodulator Compound, AS101, on Dextran Sodium Sulfate-induced Murine Colitis
    Article Snippet: Blots were developed using horseradish peroxidase-conjugated secondary antibodies and the ECL detection system (Pierce). .. A 96-well plate was coated with 80 μl/well of 5μg/ml recombinant mouse MAdCAM-1 Fc chimera (R&D Systems). .. For the in vitro assay, control wells were coated with 2.5% BSA (Sigma).

    Control:

    Article Title: Tellurium Compounds Prevent and Reverse Type-1 Diabetes in NOD Mice by Modulating α4β7 Integrin Activity, IL-1β, and T Regulatory Cells
    Article Snippet: .. FITC anti-mouse CD3; PE anti-human/mouse Integrin β7 (Biolegend, San Diego, CA, USA); APC anti-mouse CD45R/B220; anti-Mouse/Rat Foxp3 PE; anti-Mouse CD4 FITC; anti-Mouse CD25 APC; rat IgG2a Isotype Control PE (eBioscience, Carlsbad, CA, USA); Alexa 594 anti-mouse; Alexa 488 anti-rabbit; anti-glucagon; anti-insulin (Innovex Biosciences, Richmond, CA, USA); recombinant mouse MAdCAM-1 Fc chimera (R&D BioSystems, Minneapolis, MN, USA); murine TNF-α (Peprotech, Rocky Hill, NJ, USA); mouse IL-1β, IFNγ, and IL-17 ELISA kits (R&D Biosystems). .. AS101 and SAS were supplied by M. Albeck, Bar-Ilan University, Ramat-Gan, Israel, and were synthesized at Bar-Ilan University.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Tellurium Compounds Prevent and Reverse Type-1 Diabetes in NOD Mice by Modulating α4β7 Integrin Activity, IL-1β, and T Regulatory Cells
    Article Snippet: .. FITC anti-mouse CD3; PE anti-human/mouse Integrin β7 (Biolegend, San Diego, CA, USA); APC anti-mouse CD45R/B220; anti-Mouse/Rat Foxp3 PE; anti-Mouse CD4 FITC; anti-Mouse CD25 APC; rat IgG2a Isotype Control PE (eBioscience, Carlsbad, CA, USA); Alexa 594 anti-mouse; Alexa 488 anti-rabbit; anti-glucagon; anti-insulin (Innovex Biosciences, Richmond, CA, USA); recombinant mouse MAdCAM-1 Fc chimera (R&D BioSystems, Minneapolis, MN, USA); murine TNF-α (Peprotech, Rocky Hill, NJ, USA); mouse IL-1β, IFNγ, and IL-17 ELISA kits (R&D Biosystems). .. AS101 and SAS were supplied by M. Albeck, Bar-Ilan University, Ramat-Gan, Israel, and were synthesized at Bar-Ilan University.



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